University of Pittsburgh

Jerrod A. Poe, PhD

Research Assistant Professor





	
	


			

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Contact

    		

		jep16@pitt.edu	
		

		
			

		412-648-9240	
		

		
	
	
			

		Fax: 
		412-624-1401	
		

	    

    
    
   
    
   
    
    		

		
533 Bridgeside Point II
450 Technology Drive
	
		

	    
    
   
   
   
   
   
   
    
    
	
	
	
			

		Pittsburgh, PA 15219	
		

		
		
	



	
Education
	
	

	
PhD in Biochemistry and Molecular Genetics, University of Pittsburgh, School of Medicine, 2009
BS in Biology, Erskine College, 2003
        

              
   
  
 
 	
Research Summary
	
	

	
My research focuses on (1) The development and implementation of high-throughput/high-content screening assays for the discovery of small molecules that influence the quaternary structure of the HIV-1 Nef virulence factor.  (2) Executing the confirmatory and secondary (functional) assays required to validate potential inhibitors identified from the primary screening process.  Testing includes monitoring levels of HIV replication, infectivity, receptor downregulation and key protein-protein interactions. 
Following his graduation, Jerrod received an offer to work under his mentor, Dr. Tom Smithgall, as a Postdoctoral Associate.
	
	




  
 
 
 
 
 
 
 
 	
Mentor
	
	

	
Thomas E. Smithgall, PhD
        

                
        
        
        
        	
Dissertation
	
	

	Bimolecular Fluorescence Complementation Reveals that HIV-1 Nef Oligomerization is Essential for CD4 Downregulation and Viral Replication.	
	


        
        
        
        
 
 
   
   
















        









	
Research Lab Affiliation
	
	

	Smithgall Lab	
	













	
Publications
	
	

	
Tarafdar S, Poe J.A. and Smithgall, T.E. (2014) The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem. 289:15718-15728. |  View Abstract
Poe J.A., Vollmer L, Vogt A and Smithgall, T.E. (2014) Development and validation of a high-content bimolecular fluorescence complementation assay for small-molecule inhibitors of HIV-1 Nef dimerization. J Biomol Screen. 19: 556-565. |  View Abstract
Perrone R; Nadai M; Poe J.A; Frasson I; Palumbo M; Palu G; Smithgall T.E. and Richter, S.N. (2013) Formation of a unique cluster of G-quadruplex structures in the HIV-1 Nef coding region: implications for antiviral activity. PLoS One 8: e73121. |  View Abstract
Perrone R; Nadai M; Frasson I; Poe J.A; Butovskaya E; Smithgall T.E; Palumbo M; Palu G and Richter S.N. (2013) A dynamic G-quadruplex region regulates the HIV-1 long terminal repeat promoter. J Med Chem. 56: 6521-6530. |  View Abstract
Emert-Sedlak LA, Narute P, Shu ST, Poe JA, Shi H, Yanamala N, Alvarado JJ, Lazo JS, Yeh JI, Johnston PA and Smithgall TE. 2013. Effector kinase coupling enables high-throughput screens for direct HIV-1 Nef antagonists with antiretroviral activity. Chem Biol. 20: 82-91. |  View Abstract
Poe J.A and Smithgall T.E. (2009) HIV-1 Nef dimerization is required for Nef-mediated receptor downregulation and viral replication. J Mol Biol. 394: 329-342. |  View Abstract
Ye H; Choi H.J; Poe J and Smithgall T.E. (2004) Oligomerization is required for HIV-1 Nef-induced activation of the Src family protein-tyrosine kinase, Hck. Biochemistry. 43: 15775-15784. |  View Abstract